| Gwinnett County Public Schools | Suwannee | Public Schools | 23,300 |
| Gwinnett County Government | Lawrenceville | Local Government | 5,300 |
| Northside Hospital Lawrenceville | Lawrenceville | Hospitals, General Medical & Surgical | 3,800 |
| Primerica Inc. | Duluth | Insurance Agents, Brokers, Service | 1,791 |
| Publix Distribution & Manufacturing | Lawrenceville | Grocer Distribution | 1,600 |
| Eastside Medical Center | Snellville | Hospitals, General Medical & Surgical | 1,250 |
| Georgia Gwinnett College | Lawrenceville | Colleges, Universities | 1,127 |
| Assurant Inc. Service Center | Duluth | Insurance Carriers, Other | 950 |
| Northside Hospital Duluth | Duluth | Hospitals, General Medical & Surgical | 850 |
| Global Industrial | Buford | Industrial Machinery & Equipment | 750 |
| Price Industries | Suwanee | Sheet Metal Work | 700 |
| WIKA USA | Lawrenceville | Industrial Instruments for Measurement | 700 |
| Pruitt Health | Norcross | Offices of Holding Companies, Other | 600 |
| Boehringer Ingelheim/Merial Inc. | Duluth | Pharmaceutical Preparations | 575 |
| Atlanta Journal/Constitution | Norcross | Newspapers, Publishing and or Printing | 550 |
| Gwinnett Technical College | Lawrenceville | Colleges, Junior, & Technical Institutes | 550 |
| Sage | Lawrenceville | Prepackaged Software | 540 |
| Comcast Cable Communications Inc. | Norcross | Cable & Other Pay Television Services | 530 |
| AGCO Corp. | Duluth | Farm Machinery & Equipment | 510 |
| FleetCor Technologies Inc. | Norcross | Short-Term Business Credit | 500 |
| Pond & Co. | Norcross | Engineering Services | 500 |
